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Readers ask: What determines the order of amino acids in a protein?

The actual order of the amino acids in the protein is called its primary structure and is determined by DNA. The order of deoxyribonucleotide bases in a gene determines the amino acid sequence of a particular protein.

What determines the order of amino acids in a protein quizlet?

The amino acid sequence of a polypeptide is coded by a gene. the sequence of bases in the DNA of the gene determines the sequence of amino acids in the polypeptide.

How do you determine the order of amino acids?

There are two main methods used to find the amino acid sequences of proteins. Mass spectrometry is the most common method in use today because of its ease of use. Edman degradation using a protein sequenator is the second method, which is most useful if the N-terminus of a protein needs to be characterized.

What determines the amino acids sequence in the protein?

Even with the use of the DNA base sequence to determine primary structure, there is still a need to work with isolated proteins. The amino acid sequence deduced by reading the DNA sequence is that of the nascent protein, the direct product of the translational machinery. Many proteins are modified after synthesis.

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What determines the order of amino acids in the proteins of a cell?

DNA carries the genetic information for making proteins. The base sequence determines amino acid sequence in protein. Messenger RNA (mRNA) is a molecule which carries a copy of the code from the DNA, in the nucleus, to a ribosome, where the protein is assembled from amino acids.

How do ribosomes know which order to put amino acids in?

tRNAs bring their amino acids to the mRNA in a specific order. This order is determined by the attraction between a codon, a sequence of three nucleotides on the mRNA, and a complementary nucleotide triplet on the tRNA, called an anticodon. At the beginning of translation, the ribosome and a tRNA attach to the mRNA.

How do you determine protein sequence?

The two major direct methods of protein sequencing are mass spectrometry and Edman degradation using a protein sequenator (sequencer). Mass spectrometry methods are now the most widely used for protein sequencing and identification but Edman degradation remains a valuable tool for characterizing a protein’s N-terminus.

What determines the amino acid content and the sequence of amino acids for a specific protein?

The precise amino acid content, and the sequence of those amino acids, of a specific protein, is determined by the sequence of the bases in the gene that encodes that protein. The chemical properties of the amino acids of proteins determine the biological activity of the protein.

What organic compound determines the number and sequence of amino acids?

DNA and RNA have different functions relating to the genetic code and proteins. Like a set of blueprints, DNA contains the genetic instructions for the correct sequence of amino acids in proteins. RNA uses the information in DNA to assemble the amino acids and make the proteins.

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What determines the function of a protein?

The Rules of Protein Structure. The function of a protein is determined by its shape. The shape of a protein is determined by its primary structure (sequence of amino acids). The sequence of amino acids in a protein is determined by the sequence of nucleotides in the gene (DNA) encoding it.

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